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The actual provenance of these threads is uncertain, as Gonella was not authorized to take or retain genuine shroud material, Raymond Rogers stated in a 2005 article that he performed chemical analyses on these undocumented threads, and compared them to the undocumented Raes threads as well as the samples he had kept from his STURP work.He stated that his analysis showed: "The radiocarbon sample contains both a gum/dye/mordant coating and cotton fibers. The blind-test method was abandoned, because the distinctive three-to-one herringbone twill weave of the shroud could not be matched in the controls, and it was therefore still possible for a laboratory to identify the shroud sample.

Since the C14 dating at least four articles have been published in scholarly sources contending that the samples used for the dating test may not have been representative of the whole shroud.

The Shroud of Turin, a linen cloth that tradition associates with the crucifixion and burial of Jesus, has undergone numerous scientific tests, the most notable of which is radiocarbon dating, in an attempt to determine the relic's authenticity. Shredding the samples would not solve the problem, while making it much more difficult and wasteful to clean the samples properly.

In 1988, scientists at three separate laboratories dated samples from the Shroud to a range of 1260–1390 AD, which coincides with the first certain appearance of the shroud in the 1350s and is much later than the burial of Jesus in 30 or 33 AD. Samples were taken on April 21, 1988, in the Cathedral by Franco Testore, an expert on weaves and fabrics, and by Giovanni Riggi, a representative of the maker of bio-equipment "Numana".

and those "minute" fibers were identified as cotton by Peter South (textile expert of the Derbyshire laboratory) who said: "It may have been used for repairs at some time in the past, or simply became bound in when the linen fabric was woven.

It may not have taken us long to identify the strange material, but it was unique amongst the many and varied jobs we undertake.” The official report of the dating process, written by the people who performed the sampling, states that the sample "came from a single site on the main body of the shroud away from any patches or charred areas." Mechthild Flury-Lemberg is an expert in the restoration of textiles, who headed the restoration and conservation of the Turin Shroud in 2002.

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